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immortalized normal villous trophoblast cells sw.71  (Applied Biological Materials Inc)
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Applied Biological Materials Inc immortalized normal villous trophoblast cells sw.71
Assessment of HTLV-1 susceptibility of human primary cells in the cell-free infection model ( A ) Susceptibility of cells constituting the blood-placental barrier (HVT, HVMF, HPVEC, and HUVEC) and PBMCs to HTLV-1 in the cell-free infection model. The PVLs were analyzed at 48 hpi, and the data are the means ± SD of three independent experiments. ( B ) Relative expression of gag/pol and env , tax/rex , and HBZ mRNAs at 48 hpi were evaluated by real-time PCR in immortalized normal <t>trophoblast</t> (Sw.71), MOLT4, and Raji cells infected with HTLV-1 in the cell-free infection model. In panels A and B, asterisks represent significant differences versus the data for HVT and Sw.71, respectively (** P < 0.01 by one-way ANOVA followed by Dunnett’s multiple-comparisons test). HVT; human villous trophoblasts, HVMF; human villous mesenchymal fibroblasts, HPVEC; human placental vascular endothelial cells, HUVEC; human umbilical vein endothelial cells, PBMC; peripheral blood mononuclear cells.
Immortalized Normal Villous Trophoblast Cells Sw.71, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immortalized normal villous trophoblast cells sw.71 - by Bioz Stars, 2026-04
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Assessment of HTLV-1 susceptibility of human primary cells in the cell-free infection model ( A ) Susceptibility of cells constituting the blood-placental barrier (HVT, HVMF, HPVEC, and HUVEC) and PBMCs to HTLV-1 in the cell-free infection model. The PVLs were analyzed at 48 hpi, and the data are the means ± SD of three independent experiments. ( B ) Relative expression of gag/pol and env , tax/rex , and HBZ mRNAs at 48 hpi were evaluated by real-time PCR in immortalized normal trophoblast (Sw.71), MOLT4, and Raji cells infected with HTLV-1 in the cell-free infection model. In panels A and B, asterisks represent significant differences versus the data for HVT and Sw.71, respectively (** P < 0.01 by one-way ANOVA followed by Dunnett’s multiple-comparisons test). HVT; human villous trophoblasts, HVMF; human villous mesenchymal fibroblasts, HPVEC; human placental vascular endothelial cells, HUVEC; human umbilical vein endothelial cells, PBMC; peripheral blood mononuclear cells.

Journal: Journal of Virology

Article Title: Establishment of a novel human T-cell leukemia virus type 1 infection model using cell-free virus

doi: 10.1128/jvi.01862-23

Figure Lengend Snippet: Assessment of HTLV-1 susceptibility of human primary cells in the cell-free infection model ( A ) Susceptibility of cells constituting the blood-placental barrier (HVT, HVMF, HPVEC, and HUVEC) and PBMCs to HTLV-1 in the cell-free infection model. The PVLs were analyzed at 48 hpi, and the data are the means ± SD of three independent experiments. ( B ) Relative expression of gag/pol and env , tax/rex , and HBZ mRNAs at 48 hpi were evaluated by real-time PCR in immortalized normal trophoblast (Sw.71), MOLT4, and Raji cells infected with HTLV-1 in the cell-free infection model. In panels A and B, asterisks represent significant differences versus the data for HVT and Sw.71, respectively (** P < 0.01 by one-way ANOVA followed by Dunnett’s multiple-comparisons test). HVT; human villous trophoblasts, HVMF; human villous mesenchymal fibroblasts, HPVEC; human placental vascular endothelial cells, HUVEC; human umbilical vein endothelial cells, PBMC; peripheral blood mononuclear cells.

Article Snippet: Immortalized normal villous trophoblast cells, Sw.71 , were purchased from Applied Biological Materials (APB, Richmond, Canada) and cultured under the manufacturer's recommended conditions.

Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction